Device

Part:BBa_K2314127

Designed by: Lei Zhu   Group: iGEM17_OUC-China   (2017-10-22)


RFP Coding Device(Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm)

This is an RFP fluorescent device that can be used for fluorescence measurement experiments. The enhancement effect of UTRrpsT on the promoter was demonstrated by comparison test. Any team of interested parties can use it to do the control group on UTRrpsT. The future team can use this as a starting point to design a rich 5'UTR structure.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
    Illegal AgeI site found at 728
    Illegal AgeI site found at 840
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
emission607nm
excitation584nm